Regulatory

Part:BBa_K1679036:Design

Designed by: Xihan Zhang   Group: iGEM15_OUC-China   (2015-09-17)


pT7-100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It's the wild type T7 promoter which is stronger than mutations. It's design for our T7 protein expression device and can be substitute by the restriction site HindIII and SalI.


Source

Synthesized by Genwiz.

References

Schaerli Y, Munteanu A, Gili M, et al. A unified design space of synthetic stripe-forming networks[J]. Nature communications, 2014, 5.